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. 2021 Jul 14;12:699783. doi: 10.3389/fmicb.2021.699783

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Copyright © 2021 Sarmiento-Villamil, de Oliveira, Naruzawa and Bernier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bar graphs showing the effects of using 1 Kb or 2 Kb of homologous sequence flanking targeted loci, and applying counterselection with 5-Fluoro-2′-deoxyuridine, on the frequency of homologous recombination (HR) in Ophiostoma novo-ulmi strain 174_68Δmus52. (A) HR frequency for targeted locus ade1. (B) HR frequency for targeted locus ade7. Standard error bars are displayed at the top of each bar. Different letters above each bar indicate statistically significant differences (p < 0.05) according to the sequential Bonferroni method for error correction.