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. 2021 Jul 14;12:699783. doi: 10.3389/fmicb.2021.699783

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Copyright © 2021 Sarmiento-Villamil, de Oliveira, Naruzawa and Bernier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gel images showing PCR confirmation of ade1 or ade7 deletion in Ophiostoma novo-ulmi transformants. (A) Total genomic DNA samples from twelve pink colony transformants and one white colony transformant (t13) were amplified with ade1 specific primer pair ade1F-809 and ade1R-1221 (top panel) and nat1 marker primers ntcF_562 and ntc_975_R (bottom panel). Lanes: M, 1 kb plus (FroggaBio); t1 through t6 and t7 through 13, 174_68Δmus52 transformants obtained using pade1_NCT_2kb and pade1_NTC_1kb, respectively; PS, parental strain 174_68Δmus52; p1, pA-NTC-OSCAR; nc, negative H₂O control. (B) Total genomic DNA samples from twelve pink colony transformants and one white colony transformant (t26) were amplified with ade7 specific primer pair ade7F-368 and ade7R-809 (top panel) and nat1 marker primers ntcF_562 and ntc_975_R (bottom panel). Lanes: M, 1kb plus (FroggaBio); t14 through t19 and t20 through 26, 174_68Δmus52 transformants obtained using pade7_NTC_2kb and pade1_NTC_1kb, respectively; PS, parental strain 174_68Δmus52; p1, pA-NTC-OSCAR; nc, negative H₂O control.