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. 2021 Jul 28;16:66. doi: 10.1186/s13020-021-00474-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PTE promotes apoptosis and autophagy by downregulating ASIC2 in TGF-β1-induced cells. A549 and AECs that were transfected with ASIC2 overexpression plasmid were treated with 10 ng/ml TGF-β1 and 30 μmol/L PTE for 24 h. Apoptosis was analyzed by flow cytometry assay (A, B). The expressions of apoptosis-related protein (C, D) and autophagy-related protein (E, F) were detected by western blot. All experiments were repeated 3 times independently, and Student’s t test or ANOVA was used to compare the differences between groups. *P < 0.05 compared with TGF-β1 group; ^#P < 0.05 compared with TGF-β1 + PTE group