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. 2021 Jul 28;21:400. doi: 10.1186/s12935-021-02103-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Validation of HOTAIR as a sponge of miR-214-3p in cervical cancer cells. A Bioinformatics analysis of miR-214-3p response elements on HOTAIR sequence. The seed sequences of miR-214-3p (CAGCAGG) were fully complementary to the 1807–1813 bp on HOTAIR. B The activity of luciferase was significantly reduced after co-transfected with miR-214-3p mimics and HOTAIR reporter plasmid in HEK-293 T cells overexpressing miR-214-3p. C Knockdown of HOTAIR significantly upregulated the expression of miR-214-3p in HPV16 positive cells, but not in HPV negative C-33A cells. D HOTAIR-A (contain MRE) obviously inhibited miR-214-3p expression in C-33A cells. E The expression level of miR-214-3p was significantly upregulated when HPV16 E7 was inhibited in End1/E6E7 and SiHa cells, respectively. F The expression level of miR-214-3p was significantly reduced when HPV16 E7 expression was enhanced in C33A, End1/E6E7 and SiHa cells, respectively. The mean values and standard error were obtained from three independent experiments. *P < 0.05, ***P < 0.001