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. 2021 Jul 28;12:426. doi: 10.1186/s13287-021-02506-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The Mkx content of AMSCs and BMSCs and the mRNA and protein expressions of Mkx and tenogenic differentiation markers after downregulation of Mkx by siRNA in BMSCs. qRT-PCR analysis (a) and representative western blots (b) of Mkx content in AMSCs and BMSCs. c qRT-PCR analysis of gene expression of Mkx after siRNA application in normoxia condition. d qRT-PCR analysis of gene expression of Mkx, Col-1a1, Col-3a1, Dcn, and Tnmd in the scramble group and the siRNA group in hypoxia condition. e Representative western blots of Mkx, Col-1a1, Col-3a1, Dcn, and Tnmd in the scramble group and the siRNA group in normoxia condition and in hypoxia condition. Representative immunofluorescence staining (f) of Mkx, Col-1a1, Col-3a1, Tnmd, and quantification data (g) in the scramble group and the siRNA group in hypoxia condition. Scale bar = 50 μm; magnification, × 400. Data were shown as mean ± SD. ^★p < 0.05; ^★★p < 0.01