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. 2021 Jul 28;20:148. doi: 10.1186/s12934-021-01641-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — A general workflow employed in the current study. The chimeric promoter library was generated by randomizing the hexameric Kozak motif of the UAS_F-E-C-Core1 artificial promoter to any of the four DNA bases. Several Kozak variants with strong protein expression activity were obtained by GFP fluorescence screening. This library was then applied for metabolic pathway optimization in S. cerevisiae BY4742 strain