Strains |
|
|
BY4742 |
S288c, MATα, his3Δ1, leu2Δ0, lys2Δ0, MET15, ura3Δ0
|
Thermo Fisher Scientific |
Ypl001 ~ 004,007 ~ 008 |
BY4742 derivative, carrying plasmids YPL001 ~ 004, 007 ~ 008 |
This study |
BY4742-Kn
|
BY4742 derivatives, carrying chimeric promoter library with YLP002 as plasmid backbone |
This study |
BY4742-Cas9 |
BY4742 derivative, carrying plasmid #43,802 |
This study |
K0/528/536/540-tHMG1 |
BY4742 derivative, HMG1 was truncated in situ and tHMG was expressed using the synthetic minimal promoters K0/528/536/540
|
This study |
Plasmids |
|
|
YPL001 |
CDSgfp-TScSPG5 cassette inserted into pRS313 |
This study |
YPL002(K0) |
UASF-E-C-core11 promoter inserted into YPL001 |
This study |
YPL003/004 |
Extended/Shortened 5′UTR of K0
|
This study |
YPL007/008 |
ScTDH3/ScTEF1 promoter inserted into YPL001 |
This study |
YPL002-K528
|
YPL002 with Kozak variant “GCAATA” |
This study |
YPL002-K536
|
YPL002 with Kozak variant “CACCAA” |
This study |
YPL002-K540
|
YPL002 with Kozak variant “ATCGTC” |
This study |
p414-TEF1p-Cas9-CYC1t |
The Cas9 protein was expressed using a TEF1 promoter and a CYC1 terminator |
Addgene plasmid #43802 |
p426-SNR52p-gRNA.CAN1.Y-SUP4t |
The gRNA cassette was expressed using an SNR52 promoter and a SUP4 terminator |
Addgene plasmid #43803 |