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. 2021 Jul 28;20:148. doi: 10.1186/s12934-021-01641-z

Table 1.

Strains and plasmids used in this work

Names Characteristics Source or reference
Strains
 BY4742 S288c, MATα, his3Δ1, leu2Δ0, lys2Δ0, MET15, ura3Δ0 Thermo Fisher Scientific
 Ypl001 ~ 004,007 ~ 008 BY4742 derivative, carrying plasmids YPL001 ~ 004, 007 ~ 008 This study
 BY4742-Kn BY4742 derivatives, carrying chimeric promoter library with YLP002 as plasmid backbone This study
 BY4742-Cas9 BY4742 derivative, carrying plasmid #43,802 This study
 K0/528/536/540-tHMG1 BY4742 derivative, HMG1 was truncated in situ and tHMG was expressed using the synthetic minimal promoters K0/528/536/540 This study
Plasmids
 YPL001 CDSgfp-TScSPG5 cassette inserted into pRS313 This study
 YPL002(K0) UASF-E-C-core11 promoter inserted into YPL001 This study
 YPL003/004 Extended/Shortened 5′UTR of K0 This study
 YPL007/008 ScTDH3/ScTEF1 promoter inserted into YPL001 This study
 YPL002-K528 YPL002 with Kozak variant “GCAATA” This study
 YPL002-K536 YPL002 with Kozak variant “CACCAA” This study
 YPL002-K540 YPL002 with Kozak variant “ATCGTC” This study
 p414-TEF1p-Cas9-CYC1t The Cas9 protein was expressed using a TEF1 promoter and a CYC1 terminator Addgene plasmid #43802
 p426-SNR52p-gRNA.CAN1.Y-SUP4t The gRNA cassette was expressed using an SNR52 promoter and a SUP4 terminator Addgene plasmid #43803