Skip to main content
. Author manuscript; available in PMC: 2021 Aug 2.
Published in final edited form as: Sci Signal. 2021 Feb 2;14(668):eabc5429. doi: 10.1126/scisignal.abc5429

Figure 1. AD brains display increased eIF2α-P and reduced levels of eIF2B subunits.

Figure 1.

Cortical tissue from AD subjects and age-matched controls were probed for translation initiation factors. Samples were probed for eIF2α-P (A; F = 3.125; N = 7 Control cases; N = 7 AD cases), total eIF2α (B; F = 1.319; N = 8 Control cases; N = 8 AD cases), eIF2γ (C; F = 1.547; N = 8 Control cases; N = 7 AD cases), eIF2Bα (D; F = 1.375; N = 8 Control cases; N = 8 AD cases) and eIF2Bε (E; F = 2.823; N = 8 Control cases; N = 7 AD cases) by Western blotting (AD). eIF2α-P was normalized by total eIF2α. eIF2α, eIF2γ, eIF2Bα and eIF2Bε were normalized by GAPDH. Non-contiguous lanes from the same blot are shown in C, D and E. Outliers were excluded from each analysis using Grubb’s test (α= 0.05) from GraphPad Prism 6. *p < 0.05; **p < 0.01; Two-tailed unpaired t-test. Dots represent individual subjects.