Table 2.

Immunohistochemistry and immunofluorescence antigen retrieval, primary and secondary antibodies

Antigen retrieval	Primary antibody	Secondary antibody
10 mM sodium citrate (pH 6)	Wide spectrum screening cytokeratin (Pan-CK) (Z0622, Dako, Mulgrave, Victoria, Australia, 1:200)	Biotinylated goat anti-rabbit IgG (BA-100, Vector Laboratories, Meadowbrook, QLD, Australia, 1:150)
	Rabbit myeloperoxidase (MPO) (ab45977, Abcam, Cambridge, MA, USA, 1:100)	Donkey anti-rabbit 568 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:500)
	Rabbit CD45 (ab10558, Abcam, Cambridge, MA, USA, 1:100)	Goat anti-rabbit 647 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:500)
Tris-EDTA buffer (pH 9)	Rat anti-neutrophil (NIMP-R14) (ab2557, Abcam, Cambridge, MA, USA, 1:100)	Goat anti-rat 488 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:100)
	Rabbit recombinant anti-heme oxygenase 1 (HO-1) (ab52947, Abcam, Cambridge, MA, USA, 1:100)	Donkey anti-rabbit 568 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:500)