Fig. 4.

Elevated hydroxyl radicals in cisplatin-treated human lung cancer cells. a Augmented level of cellular ROS and hydroxyl radicals (OH^·) in lung cancer H460 cells after treatment with 50 µM cisplatin for 6 h, as determined by flow cytometric analysis with DCFH₂-DA and HPF, respectively, b without alteration of superoxide anions (O₂^·−) levels (flow cytometry chromatogram of DHE). c Detection with Amplex red clearly demonstrated the increased relative level of cellular hydrogen peroxide (H₂O₂) in H460 cells after culture with 500 µM H₂O₂, but not by 50 µM cisplatin. Data are shown as the mean ± SD (n = 3). *p < 0.05 versus untreated control cells