Fig. 6.

Effect of hydroxyl radical scavenger on cisplatin-induced toxicity in human lung cancer cells. a Pretreatment with 1 mM deferoxamine, a hydroxyl radical scavenger, for 30 min prior exposure with 50 µM cisplatin for 24 h significantly reduced cell viability in lung cancer H460 cells compared with the cells only treated with cisplatin. b The increment of percentage of apoptotic cells was remarkably observed in H460 cells pretreated with 1 mM deferoxamine in response to cisplatin treatment. c Correspondingly, costaining with Hoechst33342/propidium iodide (PI) clearly demonstrated the accumulation of apoptotic cells presenting with bright blue fluorescence of Hoechst33342 without PI red fluorescence-stained necrotic cells in deferoxamine-pretreated H460 cells compared with both untreated control and only cisplatin treated cells. Data are shown as the mean ± SD (n = 3). *p < 0.05 versus untreated control cells. ^#p < 0.05 versus only cisplatin-treated cells