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. 2021 Jul 28;21:863. doi: 10.1186/s12885-021-08561-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Aneuploid pleomorphic sarcoma cells are sensitive to AICAR treatment. A Growth of IB105 DsRed, IB106 GFP and IB105/106 hybrids in absence or presence of AICAR treatment (700 μM for 6 days) evaluated by flow cytometry. For each cell line, data are expressed as growth percentage compared to the growth of the untreated sample (100%). Statistical tests were done using a t-test (nonparametric) Mann-Whitney (**p < 0.01, error bars, SD; n = 5 done in sextuplicates twice and in triplicates for the rest of the experiments). B Migration of IB105 DsRed, IB106 GFP and IB105/106 cells post-vehicle or AICAR pre-treatment (700 μM for 24 h) evaluated by wound healing assays using IncucyteS3. Data represent relative wound density at 8 or 24 h post-scratch. Statistics were done using a t-test. (Nonparametric) Mann-Whitney (***p < 0.001, ****p < 0.0001, error bars, SD; n = 2 done in 12 wells for each sample, condition and per experiment)