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. 2021 Jul 28;19:224. doi: 10.1186/s12951-021-00966-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — NPC targeting ability and cytotoxicity of Cur@α-NTP-LNs. a Confocal images of 5-8F cells incubated with 10 µM Cur@α-NTP-LNs and free Cur. Scale bar = 20 µm. b Quantitative flow cytometry analysis of the fluorescence intensity of Cur in 5-8F cells treated with a serial concentration of Cur@α-NTP-LNs and free Cur for 3 h (n = 4). c, d Proliferation assay evaluating the cytotoxic effect on 5-8F cells of a serial Cur concentration of c free Cur and Cur@α-NTP-LNs or a serial peptide concentration of d Cur@α-NTP-LNs and α-NTP-LNs for 24 h (n ≥ 3). Results are presented as mean ± s.d. ^***p < 0.001 and ^*p < 0.05