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. 2021 Jul 28;19:224. doi: 10.1186/s12951-021-00966-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Evaluation of Cur targeted delivery of Cur@α-NTP-LNs in vivo. a UV–visible absorption spectrum of Cur(DiR-BOA)@α-NTP-LNs, free Cur, and free DiR-BOA in PBS solution at room temperature. b Blood circulation of Cur(DiR-BOA)@α-NTP-LNs in normal nude mice (n = 5). c Whole-body imaging of 5-8F tumor-bearing mice at 24 and 48 h after intravenous injection of Cur(DiR-BOA)@α-NTP-LNs. d ex vivo images of the tumor and normal organs (brain, muscle, kidney, lung, spleen, liver, and heart) after imaging. e Confocal imaging analysis of the co-localization of Cur (Green) and DiR-BOA (Red) in tumor sections pre-stained with DAPI (blue) before multispectral microscopy. Scale bar = 50 µm. Results are presented as mean ± s.d