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. 2021 Jul 27;21:45. doi: 10.1186/s12896-021-00707-5

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© The Author(s) 2021

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Fig. 2 — Ribonucleoprotein (RNP) complex, the proximity of the cut to the edit site, and inclusion of a silent mutation increase efficiency of single base pair edits. A Design of gRNA (green), PAM (orange), silent mutation (purple box) in respect to target T55 location (red box). B, C Locations and types of mutations in the first round of RNP (RNP 1, green) and the second round of RNP (RNP 2, yellow) of editing. Scissors indicate Cas9 cut site. D Chromatogram of Wildtype (top), heterozygous (middle), and homozygous (bottom) T55A mutations. Note data represents sequence data of template strand