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. 2021 Jul 28;12:427. doi: 10.1186/s13287-021-02507-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Effects of LPS-pre EVs on protein expression levels in chondrocytes. a, b Protein expression of aggrecan, COL2A1, and ADAMTS5 in chondrocytes of each group analyzed by western blot (n = 3, one-way ANOVA). c, d Protein expression of aggrecan, COL2A1, and ADAMTS5 in chondrocytes of each group analyzed by immunofluorescence staining (n = 3, one-way ANOVA). e, f Protein expression of aggrecan, COL2A1, and ADAMTS5 in chondrocytes treated with different concentrations of LPS-pre EVs and analyzed by western blot (n = 3, one-way ANOVA). b, d, f Values are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA