Figure 7:

Role of actin in Huh 7 cell mechanoresponse. (A) Bright-field image of Huh7 cell plated on elastic (upper panel) and viscoelastic (lower panel) substrate when cells are treated with Latrunculin A at 0hr and 4 hrs. time. Scale bar 100 μm. (B) Time evolution of cell area (top) and roundness (bottom) on elastic (blue) and viscoelastic (red) substrates. Error bars represent standard error mean (N=15 cells on each substrate). (C) Huh7 cell area at 4 hrs. (left) and fold change in area (right) with respect to control when treated with Lat A on elastic (blue) and viscoelastic (red) substrate shown. Error bars represent standard error mean (N=15 cells on each substrate). p-values are determined from Student’s t-test for unpaired samples f (** p<0.005; * p<0.05).