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. 2021 Jul 14;12:682767. doi: 10.3389/fphar.2021.682767

FIGURE 2.

FIGURE 2

Efficacy of PER monotherapy in hyperthermia-induced seizure Scn1a E1099X/+ mice. (A) Schematic illustrations of the protocol of the hyperthermia-induced seizure experiments. (B) Representative EEG trace (3.9–7.5 Hz) of a spontaneous seizure in vehicle and PER treatment groups (n = 6 per group) under different hyperthermia. (C) Percentage of mice remaining free of seizure plotted against core temperature. p = 0.012, Het vs. Het PER 1 mg/kg; p = 0.0005, Het vs. Het PER 2 mg/kg. p-Values were determined by logrank Mantel–Cox tests followed by Bonferroni’s post hoc comparison tests with n = 6 per treatment group. (D) Quantitative results of temperature, seizure duration, and Racine score in the vehicle group, 1 mg/kg PER treatment group, and 2 mg/kg PER treatment group (n = 6 per group). ***p < 0.001, vehicle vs. Het PER 2 mg/kg in seizure duration; ****p < 0.0001, vehicle vs. Het PER 1 mg/kg and vehicle vs. Het PER 2 mg/kg in temperature. One-way ANOVA followed by Tukey’s multiple comparisons post hoc analysis. **p < 0.01, vehicle vs. Het PER 2 mg/kg in Racine score. The Kruskal–Wallis test. (E) The ictal discharge frequency and duration of the vehicle group (n = 3) and 2 mg/kg PER treatment group (n = 3) are illustrated. *p < 0.05, vehicle vs. Het PER 2 mg/kg in duration; **p < 0.01, vehicle vs. Het PER 2 mg/kg in frequency. Student’s unpaired t-test.