FIGURE 3.

Perampanel exhibits effective antiepileptic activity in hyperthermia-induced seizures in Scn1a ^E1099X/+ mice. Cross-over experiments were designed. (A) Mice were divided into two groups (Group1 and Group2, n = 3 per group), and temperature change, seizure duration, and Racine score were recorded for 3 weeks. Empty circles and squares represent the vehicle control group, and shaded circles and squares represent mice treated with PER 2 mg/kg. (B) Oral administration and intraperitoneal injection of PER 2 mg/kg were evaluated, and temperature change, seizure duration, and Racine score were determined in the vehicle group (n = 3) and 2 mg/kg PER treatment group (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001: vehicle vs. Het PER 2 mg/kg. Student’s unpaired t-test in temperature change and seizure duration. (C) NBQX (30 mg/kg), an AMPA receptor antagonist, was utilized for comparison of effectiveness with PER (2 mg/kg) as a positive control. The results of temperature change, seizure duration, and Racine score with PER (n = 3) and NBQX (n = 3) were similar. *p < 0.05, ***p < 0.001: vehicle vs. Het PER 2 mg/kg and vehicle vs. NBQX 30 mg/kg. One-way ANOVA followed by Tukey’s multiple comparisons post hoc analysis in temperature and seizure duration. The Kruskal–Wallis test in Racine score.