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. 2021 Jul 14;12:664385. doi: 10.3389/fmicb.2021.664385

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Copyright © 2021 Fang, Zhang, Wang, Duan, Yu, Jia, Pang, Ma, Wang and Nan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hierarchical clustering of 80 alfalfa varieties based on variations in pathogen effect sizes of all plant traits single and co-inoculated with Fom and Rs. (A) Single inoculation with Fom (Fom), (B) single inoculation with Rs (Rs), and (C) co-inoculation with Fom and Rs (Fom:Rs). Labels on the dendrogram (I, II, III, and IV) represent the main groups. The heat map shows variations in pathogen effect sizes of different traits. The color gradient range (0–100%) is shown on the top right corner. Positive effects (effect size < 0) of pathogen treatment on some traits (Supplementary Figures 1–4) were defined as zero to maximize resolution. DIS and DIR, disease index of plant shoot and root, respectively; PH, plant height; RL, root length; DWS and DWR, dry weight of shoot and root, respectively; ST, shoot biomass ratio; RT, root biomass ratio.