FIGURE 1.

Factors affecting the length of the lag phase of E. coli BW25113 and BL21(DE3). (A) Different carbon sources; cells grown in LB broth were washed and transferred to M9 minimal medium containing D-glucose or succinate. (B) Different preculture medium; cells grown in LB and M9 succinate medium were washed and transferred to M9 succinate medium. (C) Different inoculum size; cells grown in LB broth were washed and transferred to M9 succinate medium. NC, no cells were inoculated. The unit of specific growth rate (μ) is h^–1.