FIGURE 2.
The growth profile of the BL21(DE3) cells during continuous transfer to fresh M9 succinate medium and the analysis of the size of colonies from the cultures. (A) Accelerated cell growth by serial seed transfer to fresh succinate medium. The unit of specific growth rate (μ) is h– 1. (B) The occurrence of large colonies and the increase in the number of large colonies from the cultures. (C) The ratio of the number of large to small colonies that grew after spread plating the culture samples.
