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. 2021 Jul 14;12:693464. doi: 10.3389/fmicb.2021.693464

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Copyright © 2021 Kim, Jeong and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the HK565 cells adapted in succinate minimal medium, compared to the BW25113 and parental BL21(DE3) strains. (A) Growth profiles of the HK565, BW25113, and BL21(DE3) strains. The unit of specific growth rate (μ) is h^{– 1}. (B) Nucleotide sequence alignment of the promoter region of the kgtP gene in the HK565, BW25113, and BL21(DE3) strains. The nucleotides that evolved from C to T at the – 8 position of HK565 are shown in black. Unconserved nucleotide sequences are shaded in gray. (C) RT-qPCR analysis of the expression levels of kgtP in cells grown in M9 medium containing succinate or α-ketoglutarate.