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Effect of the – 8C-to-T single point mutation at the promoter of the kgtP gene on cellular growth in M9 minimal medium containing succinate or α-ketoglutarate. (A) kgtP(–8C →T)/BL21(DE3), (B) kgtPWT/BL21(DE3), (C) kgtP(–8C →T)/HK565, and (D) kgtPWT/HK565. kgtP(– 8C →T) and kgtPWT were transferred via P1 phage transduction to generate mutant cells from BL21(DE3) and revertant cells from the HK565 strain, respectively. The unit of specific growth rate (μ) is h– 1.
