TABLE 1.
Bacterial strains, phage, and plasmids used in this study.
| Name | Relevant genotypes and characteristics | References or sources |
| Strains | ||
| BW25113 | E. coli K-12, Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– rph–1 Δ(rhaD-rhaB)568 hsdR514 | CGSC |
| BL21(DE3) | F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Invitrogen |
| JW5409 | BW25113, ΔyfiP:: KmR | Keio collection |
| HK565* | BL21(DE3), kgtP(–8C→T) | This study |
| HK567* | BL21(DE3), ΔkgtP::KmR | This study |
| HK569* | BL21(DE3), ΔkgtP::KmR, dcuSrev | This study |
| HK651 | BL21(DE3), kgtPWT ΔyfiP::KmR | This study |
| HK652 | BL21(DE3), kgtP(–8C→T) ΔyfiP::KmR | This study |
| HK654 | HK565, kgtPWT ΔyfiP::KmR | This study |
| HK655 | HK565, kgtP(–8C→T) ΔyfiP::KmR | This study |
| HK1020 | BL21(DE3), kgtPWT-GGGGS linker-sfGFP | This study |
| HK1022 | HK565, kgtP(–8C→T)-GGGGS linker-sfGFP | This study |
| Phage | ||
| P1 vir | vir mutations | S. Adhya |
| Plasmids | ||
| pCP20 | Temperature-sensitive plasmid with an FLP recombinase capable of recognizing the FRT sequence, ApR | CGSC |
| pKD46 | Temperature-sensitive plasmid with lambda recombinase inducible by L-arabinose, ApR | CGSC |
| pJET1.2 | Cloning plasmid, ApR | Thermo Fisher Scientific |
*Whole-genome sequencing was performed to identify a point mutation (− 8C→T) in the kgtP gene.