Figure 2.

NETs increase in a sepsis-induced lung injury model. (A) Lung tissues were collected from C57BL/6 mice in the control group, sham group, ARDS group, or ARDS+DNase group. H&E staining was performed on paraffin-embedded sections of mouse lungs to detect lung injury. (B) Changes in the lung wet/dry ratio in the four groups (C). Changes in PaO₂ as the processing time increased in the four groups. (D) Immunofluorescence of lung tissue in red (MPO) and blue (DAPI). (E) Fluorescence imaging of mouse peripheral blood neutrophils among the healthy control, sham, ARDS, and ARDS+DNase groups. The sections were immunostained with MPO (red) and CitH3 (green), and DAPI (blue) was used to counterstain the nuclei. (F). Ex vivo plasma DNA. (G) MPO-DNA complex. (H) NET-releasing cell count assay. (I) NET degradation among the healthy control, sham, ARDS, and ARDS+DNase groups (n=6). P < 0.05 (*) and P < 0.05 (^#) compared to baseline healthy controls and the sepsis group were considered statistically significant.