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. 2021 Jul 14;11:677902. doi: 10.3389/fcimb.2021.677902

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Copyright © 2021 Zhang, Zhou, Qu, Yu, Chen, Zhu, Guo, Chen and Miao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activated platelets for subsequent NET generation in PMNs. (A) Neutrophil extracellular trap formation by control polymorphonuclear neutrophils incubated with control platelets pretreated with plasma obtained from the 3 groups. (B) Fluorescence-activated cell sorting analysis of Annexin V on control platelets treated with plasma obtained from the 3 groups (n=6). (C) Fluorescence-activated cell sorting analysis of CD62P on control platelets treated with plasma obtained from the 3 groups (n=6). (D) Percentage of NET-releasing polymorphonuclear neutrophils of control polymorphonuclear neutrophils treated with platelets and plasma obtained from the 3 groups (n=6). P < 0.05 (*) and P < 0.05 (^#) compared to baseline healthy controls and the sepsis group were considered statistically significant.