TABLE 1.
Mutants | Forward primer (5′->3′) | Reverse primer (5′->3′) |
---|---|---|
N436A | ctcttcttcctgacgaccGCccagtgtttcagcagtgt | acactgctgaaacactggGCggtcgtcaggaagaagag |
F439A | tgacgaccaaccagtgtGCcagcagtgtttcagccg | cggctgaaacactgctgGCacactggttggtcgtca |
F439W | tgacgaccaaccagtgttGGagcagtgtttcagccg | cggctgaaacactgctCCaacactggttggtcgtca |
F439Y | tgacgaccaaccagtgttAcagcagtgtttcagccg | cggctgaaacactgctgTaacactggttggtcgtca |
Capital letters indicate substitution.