REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FITC-conjugated mouse anti-CD150a (PDGFRA)	Thermo Fisher	11–1401-82; RRID: AB_2572476
Chemicals, peptides, and recombinant proteins
Tamoxifen	Sigma-Aldrich	T5648
Sunflower seed oil	Sigma-Aldrich	S5007
Insulin	Sigma-Aldrich	I1882
Rosiglitazone	Cayman Chemicals	71740
3-Isobutyl-1-methylxanthine	Sigma-Aldrich	I5879
Dexamethasone	Sigma-Aldrich	D4902
Fluoromount-G	Southern Biotech	0100
SYBR Green PCR master mix	Thermo Fisher	4312704
Z-fix Concentrate	Anatech Ltd	171
BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene)	Invitrogen	D3922
Hoechst 33342, Trihydrochloride, Trihydrate	Invitrogen	H3570
0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate	Corning	25053CI
Collagenase type II	Sigma-Aldrich	C6885
Triton X-100 Surfact-Amps	Thermo Fisher	28314
Trizol	Thermo Fisher	15596018
Critical commercial assays
RNeasy MinElute Cleanup Kit	QIAGEN	74204
Deposited data
Mouse adipose SVF single-cell RNA-seq dataset	Single cell atlas portal	https://singlecell.broadinstitute.org/single_cell/study/SCP708/mouse-adipose-stromal-vascular-fraction
Experimental models: Organisms/strains
Mouse: Krt19CreERT: Krt19tm1(cre/ERT)Ggu/J	The Jackson Laboratory	026925
Mouse: Wt1CreERT2: Wt1tm2(cre/ERT2)Wtp/J	The Jackson Laboratory	010912
Mouse: mTmG: B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo/J	The Jackson Laboratory	007676
Mouse: tdTomato: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J	The Jackson Laboratory	007909
Oligonucleotides
qPCR primers	See Table S1	Thermo Fisher
Software and algorithms
FlowJo v 10.7.1	BD	N/A
Seurat v 3.0.3.9036	Stuart et al., 2019	N/A
Zen Black v 2.3	Zeiss	N/A
Other
SORP FACSAria III	BD	N/A
LSM 880 Upright Laser Scanning Confocal Microscope	Zeiss	N/A
Ultra-Low Attachment Surface Plate, 96 well	Corning	4515