TABLE 1.

ACE, Chao1, Shannon, and Simpson indices of intestinal bacteria in different groups of houseflies.

Sample ID	Reads	OTU	Biological index
			Ace	Chao	Shannon	Simpson	Coverage
Wa	62350	625	719.58 ± 121.88	689.00 ± 111.32	3.17 ± 0.56	0.14 ± 0.09	99.80%
Ya	69331	585	732.75 ± 147.22	710.00 ± 143.32	3.00 ± 0.44	0.14 ± 0.07	99.77%
Yb	67279	629	781.58 ± 158.39	750.83 ± 153.15	3.13 ± 0.46	0.14 ± 0.1	99.75%
Yc	66343	657	832.08 ± 116.04	790.00 ± 106.33	3.14 ± 0.18	0.12 ± 0.04	99.73%
Yd	62888	805	938.50 ± 117.45	907.92 ± 123.23	3.37 ± 0.33	0.10 ± 0.05	99.74%
Ye	59266	1038	1126.33 ± 144.63	1101.83 ± 140.71	3.53 ± 0.64	0.12 ± 0.08	99.78%

Alpha diversity indices were analyzed using one-way ANOVA to characterize the microbial richness and diversity of the samples fed different dilutions of P. aeruginosa. Significance analysis was performed using Tukey’s test (p < 0.05). Wa, Ya, Yb, Yc, Yd, and Ye represent housefly larvae samples fed diets of sterile water, P. aeruginosa stock solution, and the stock solution diluted 10², 10⁴, 10⁶, and 10⁸ fold, respectively. Each treatment included three biological replicates. The error estimate represents the standard error of the mean.