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. 2021 Jul 14;12:668778. doi: 10.3389/fmicb.2021.668778

TABLE 3.

Observed values and descriptive statistics for the microbial concentrations of the Danube from 2010 to 2015 (Cr in Equation 1) and the Kruskal–Wallis test results showing that the simulated and observed values were not significantly different (p ≥ 0.05).

Parameter Observed dataset detected/n Observed median (minimum, maximum) [particles/L] Distribution Descriptive statistical parameter values gamma distribution: shape/location/scale; normal distribution: mean/standard deviation Kruskal–Wallis p Comment
Human MST marker 50/62 5.39 × 103 (60, 1.41 × 106) Gamma 0.23 / 58 / 80,000 0.05 After model optimization based on OF (Equation 14)
Ruminant MST marker 21/60 250 (60, 4.54 × 104) Gamma 0.23 / 58.5 / 10,766 0.14
Pig MST marker 14/60 220 (60, 1.58 × 105) Gamma 0.25 / 58.5 / 3,700 0.11
Bird MST marker 16/23 1.21 × 104 (60, 2.19 × 105) Gamma 0.45 / 58 / 20,000 0.05
E. coli 64/64 570 (20, 4.9 × 104) Gamma 0.19 / 100 / 27,000 0.33
Giardia 21/31 0.8 (0.2, 4.4) Normal 1.39 / 1.17 0.45 stats fit function in Python 3.7
Cryptosporidium 19/31 0.8 (0.2, 6.44) Normal 1.29 / 1.42 0.40

Non-detects were replaced by half of the TOD (MST markers) or half of the LOD (E. coli, Giardia, and Cryptosporidium).