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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Biochem J. 2020 Oct 16;477(19):3839–3850. doi: 10.1042/BCJ20200623

Figure 1. Vector maps for the expression plasmids and their co-expression in E. coli host cells.

Figure 1.

(A) The pGM expression plasmid contains the genes for ampicillin resistance and α- and β-globin cloned tandemly with a copy of the MAP gene. (B) The pMAP expression plasmid contains the kanamycin resistance gene and an additional copy of the MAP gene [47]. (C) The pNatA plasmid contains the gene that confers chloramphenicol resistance and the naa10 and naa15 genes of the NatA complex [49,50]. (D) Schematic diagram showing the co-expression of pGM + pMAP plasmids in E. coli host cells. (E) Co-expression of pGM + pMAP + pNat plasmids, with a depiction of MAP and pNatA enzyme complex during translation. The pNatA complex facilitates acetylation of the NH2-termini of alanine, valine, serine, glycine, cystine, and threonine.