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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Biochem J. 2020 Oct 16;477(19):3839–3850. doi: 10.1042/BCJ20200623

Figure 3. O2-binding properties of the native and recombinant Hbs of alligator and human.

Figure 3.

O2-affinities (P50) in the absence (stripped) and presence of anionic effectors (Cl− ions and organic phosphates) are shown for native Hbs, unacetylated rHbs (produced using the pGM + pMAP plasmid), and acetylated rHbs (produced using the pGM + pMAP + pNatA plasmid). ATP and DPG were used as physiologically relevant organic phosphates in experiments using alligator and human Hbs, respectively.