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. 2021 Jul 14;12:712614. doi: 10.3389/fimmu.2021.712614

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Copyright © 2021 Brosseau, Selle, Duval, Misme-Aucouturier, Chesneau, Brouard, Cherbuy, Cariou, Bouchaud, Mincham, Strickland, Barbarot and Bodinier

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of GOS/inulin supplementation on the concentrations of amniotic fluid metabolites. (A) Two-dimensional PCA score plot of 1H NMR integrated spectra of amniotic fluid samples (circle: control, n = 8; triangle: supplementation, n = 8). (B) Two-dimensional PLS-DA score plot of OSC-filtered and Pareto-scalde 1H NMR integrated spectra of amniotic fluid samples (circle: control, n = 8; triangle: supplementation, n = 8). (C) Boxplot of discriminant (VIP ≥ 1.0) and significant (FDR-corrected p-value ≤ 0.05) features: comparison of the median concentrations of butyrate and acetate between the control (white) and prebiotic-supplemented (gray) groups. (D) Table of discriminant metabolites in the amniotic fluid of dams fed a control or prebiotic diet. **p < 0.01.