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. 2021 Jul 14;12:712614. doi: 10.3389/fimmu.2021.712614

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Copyright © 2021 Brosseau, Selle, Duval, Misme-Aucouturier, Chesneau, Brouard, Cherbuy, Cariou, Bouchaud, Mincham, Strickland, Barbarot and Bodinier

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of SCFA (butyrate, propionate and acetate) and GOS/inulin treatment in vitro on human IL-10⁺ Breg cell and CD27⁺CD38^high plasmablast frequencies. Human B cells isolated from PBMCs were cultured for 3 days with GOS/inulin, butyrate, propionate, acetate or no treatment as a control (n=11). The frequencies of IL-10⁺ B cells and plasmablast CD27⁺CD38^high B cells were estimated by flow cytometry (* = p<0.05, ** = p<0.01, *** = p<0.001).