Skip to main content
. 2021 Jul 14;12:682603. doi: 10.3389/fmicb.2021.682603

FIGURE 4.

FIGURE 4

Amino acid alignment of ORFs encoding accessory proteins. (A) Amino acid alignment of ORF3a and 3b sequences of SARS-CoV-1 (AY274119), Bat-SL-CoV-Rp3 (DQ071615), SARS-CoV-2 (NC_045512), Bat-SL-CoV-RaTG13 (MN996532), Bat-SL-CoV-RmYN02 (JX993988), Pangolin-CoV-2019 (MT121216). No ORF3b is found in the Bat-SL-CoV-RmYN02 and Pangolin-CoV-2019 sequences, the stars indicate the stop codons. Amino acid alignment of ORF3c and 3d sequences. ORF3d is only found in the SARS-CoV-2. The asterisks indicate the stop codons. (B) Amino acid alignment of ORF6, 7a and 7b sequences. (C) Amino acid alignment of ORF8, 9b, 9c, and 10 sequences. The SARS-CoV-1 ORF8 went through a gradual deletion over the course of the epidemic and at the end of the outbreak ORF8 was divided in 2 ORFS (ORF8a and 8b). ORF10 is only found in SARS-CoV-2, Bat-SL-CoV-RaTG13 and Pangolin-CoV-2019. Sequences were analyzed using Unipro UGENE: a unified bioinformatics tollkit Okonechnikov; Golosova; Fursov. Bioinformatics 2012 28: 1,166–1,167. Amino-acids are color-coded according Clustal X. For each ORF, the SARS-CoV-2 sequence (NC_045512) was used as a reference sequence to perform the alignment.