Skip to main content
. Author manuscript; available in PMC: 2021 Aug 11.
Published in final edited form as: Immunity. 2021 Apr 9;54(5):931–946.e11. doi: 10.1016/j.immuni.2021.03.020

Figure 2. CNS0 deficiency impairs Treg cell differentiation.

Figure 2.

A-D. Frequencies of Foxp3-GFP+ (A) and CD25+Foxp3-GFP− cells (B) among CD4+CD8− TCRβ+ cells from thymuses, and Foxp3-GFP+ among CD4+TCRβ+ cell from spleens (C) of CNS0Δ and CNS0FL male littermates of indicated ages. R2 for linear regression of log-transformed fluorescence intensities of CD25 and Foxp3-GFP among CD25+Foxp3-GFP+ CD4SP thymocytes (D). Each point indicates 1 mouse and data are pooled from 5 independent experiments, including a single experiment where mice of both genotypes from all ages were analyzed.

E, F. Model of the effect of CNS0 deficiency on Treg cell induction and expansion in neonatal (E) and adult (F) mice. Cells with green nucleuses represent Treg cells and those with red membranes represent antigen experienced Tconv cells. Faded IL-2 indicates its consumption by Treg cells.

Two-way ANOVA with Sidak test to correct for multiple comparisons. p >0.05 ns, <0.05 *, <0.01 **, <0.001 ***, <0.0001 ****.

See also Figure S2.