Figure 4. Immune perturbation but no overt pathology in adult CNS0-deficient mice.

A, E, G. Frequencies of Foxp3-GFP⁺ cells (A) and CD44^hiFoxp3-GFP⁻ (E) among CD4⁺TCRβ⁺ cells, and IFNγ⁺ cells among Foxp3-GFP⁻CD4⁺TCRβ⁺ cells (G) in indicated tissues (pLN: pooled brachial, axillary, and inguinal lymph nodes; mLN: pooled mesenteric lymph nodes) from 8- to 10-week-old CNS0^Δ and CNS0^FL male littermates. Each point indicates 1 mouse and data are pooled from 2 independent experiments.

B-D. Frequencies of CD44^hiCD62L⁻ cells (B), CTLA-4 gMFI, (C) and frequencies of Ki67⁺ cells (D) among Treg cells in indicated tissues from 8- to 10-week-old CNS0^Δ and CNS0^FL male littermates. n=9 per group.

F. Serum IgG2b quantification by ELISA of CNS0^Δ and CNS0^FL male littermates of indicated ages. Each point indicates 1 mouse. Data represent 2 independent experiments.

H. Body weight of 8- to 10-week-old CNS0^Δ and CNS0^FL male littermates.

Unpaired t-tests with correction for multiple comparisons using the Holm-Sidak method. p >0.05 ns, <0.05 *, <0.01 **, <0.001 ***, <0.0001 ****.

See also Figure S4.