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. 2021 Jul 3;12(17):5345–5354. doi: 10.7150/jca.60543

Figure 3.

Figure 3

FHL1 negatively regulates the Wnt/β-catenin pathway. A-B. The p-GSK, GSK and β-catenin protein levels were measured by Western blot after FHL1 knockdown with siRNA-3 or FHL1 overexpression with Lv-GFP+FHL1 in HCT-116 cells and HT-29 cells, respectively. C-D. Real-time PCR analysis of the expression levels of LEF1 and TCF4 in FHL1 knockdown or overexpression cells. E. The LEF1 and TCF4 protein levels were assessed by Western blot after using FHL1 siRNA-3 or/and PRI-724 in HCT-116 cells. Data are presented as mean ± SD and are representative of 3 independent experiments. * P < 0.05, ** P < 0.01.