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. 2021 Jul 3;12(17):5345–5354. doi: 10.7150/jca.60543

Figure 4.

Figure 4

The effect of FHL1 overexpression or knockdown on cell cycle proteins in colorectal cancer cells was elucidated. A. The proliferation of HCT-116 cells after FHL1 knockdown by siRNA-3 or treatment with PRI-724 was measured using a CCK-8 cell-counting kit. B-C. Expression of cell cycle-related proteins was measured by Western blot after FHL1 knockdown with siRNA-3 or FHL1 overexpression with Lv-GFP+FHL1 in HCT-116 cells and HT-29 cells, respectively. Data are presented as mean ± SD and are representative of 3 independent experiments. * P < 0.05, ** P < 0.01.