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. 2021 Jul 3;12(17):5310–5319. doi: 10.7150/jca.57711

Figure 2.

Figure 2

Trop2 induced gefitinib resistance in NSCLC. (A-B) The cell viability of NSCLC cell lines with gefitinib treatment, which were been knock down (A) or over-expression (B) of Trop2. Mean ± SD, ** P < 0.01. (C-D) Trop2 expression was tested in NSCLC cell lines (PC-9) and gefitinib drug-resistance cell lines (PC-9/GR) through western blotting (C) and qRT-PCR (D), Mean ± SD, **P<0.01. (E) Cell viability was detected in PC-9/GR cells, harboring control (shNC), silencing of Trop2 (shTrop2-7), vector, or over-expression of Trop2 (OE-Trop2) at 0 h, 24 h, 48 h and 72 h with gefitinib treatment. Mean ± SD, **P<0.01, means compared with shNC group, ##P<0.01, means compared with vector group. (F-G) Migration assay of PC-9/GR cells transduced with shTrop2-7 or OE-Trop2 plasmid companied with gefitinib treatment at 24 h. Representative images of cell migration were shown (F), and (G) is the statistical graph of (F), Mean ± SD, *P<0.05, **P<0.01.