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. 2021 Jul 3;12(17):5310–5319. doi: 10.7150/jca.57711

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Trop2 physically bound with IGF2R. (A) Trop2 was over-expressed in PC-9/GR cells and the mRNA levels of the interacting proteins were detected by qRT-PCR. (B) 293T cells were transfected with HA-Trop2 or control plasmid HA-PCMV and Flag-IGF2R plasmid, and the cell lysates were immunoprecipitated with anti-flag antibody. (C) 293T cells con-transfected with Flag-IGF2R or control plasmid Flag-pcDNA3.1 and HA-Trop2 were coimmunoprecipitated by immunobloting with anti-HA-antiboby. (D) PC-9/GR cell lysates were immunoprecipitated with anti-Trop2 antibody and the coimmunoprecipitated IGF2R was detected using anti-IGF2R antibody.