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. 2021 Jul 3;12(17):5310–5319. doi: 10.7150/jca.57711

Figure 4.

Figure 4

Trop2 bound with IGF2R to activate IGF2-IGF1R-Akt axis in NSCLC gefitinib resistance. (A) Cells were transfected with shNC, shTrop2, and/or shIGF2R plasmids respectively with gefitinib treatment for 72 h to detect the proliferation ability. Mean ± SD, **P<0.01, means compared with shNC group, ##P<0.01, means compared with PC-9/GR shTrop2 group. (B) Cells were transfected with vector, OE-Trop2, and/or shIGF2R plasmids respectively for 72 h to detect the proliferation ability. Mean ± SD, **P<0.01, means compared with vector group, ##P<0.01, means compared with PC-9/GR OE-Trop2 group. (C) PC-9/GR was knocked down or over-expressed with shIGF2R or linstinib for 24 h, Western blotting was used detect the protein level of p-IGF1R, Akt and p-Akt. (D-G) The migration of cells were detected through migration assay, and the representative images of cell migration were shown in (D) and (F), (E) is the statistical graph of (D), and (G) is the statistical graph of (F), Mean ± SD, *P<0.05, **P<0.01.