Table 2.
†Endogenous concentration of five KP metabolites in the commercial human plasma sample.
| AA nM | AA ng/mL | KA nM | KA ng/mL | QA nM | QA ng/mL | TRP μM | TRP μg/mL | KYN μM | KYN μg/mL | |
|---|---|---|---|---|---|---|---|---|---|---|
| day 1 | 80 | 10.9 | 51 | 9.6 | 250 | 42 | 50.9 | 10.39 | 1.20 | 0.25 |
| day 2 | 86 | 11.8 | 44 | 8.3 | 270 | 45 | 49.9 | 10.19 | 1.08 | 0.23 |
| day 3 | 85 | 11.6 | 48 | 8.7 | 310 | 52 | 50.2 | 10.25 | 1.16 | 0.24 |
| Average | 83 | 11.4 | 48 | 8.7 | 277 | 46 | 50.3 | 10.28 | 1.15 | 0.24 |
| SD | 3 | 0.4 | 3 | 0.5 | 25 | 4 | 0.4 | 0.08 | 0.05 | 0.01 |
| %RSD | 3 | 3 | 6 | 6 | 9 | 9 | 1 | 1 | 4 | 4 |
†
The endogenous concentrations are the naturally produced amounts of specific metabolites per unit volume of human plasma (see experimental Section 2.6). Example calculations for endogenous concentration measurement are shown in the supplementary Fig. S7 (Text box B).