Figure 2.

Optical images of phagolysosome penetration using a platinized nanotip shown (a–c); (a) the tip, shown in the blue circle, is close to the macrophage, shown in the red circle; (b) the nanotip is physically contacting the cell membrane above the targeted phagolysosome (red circle); (c) the nanotip is seen inside the phagolysosome. ROS/RNS measurements inside a phagolysosome of the activated RAW 264.7 macrophage (d–f); (d) time variations of chronoamperometric currents measured at different potentials at the tip inside a phagolysosome; (e) corresponding time variations of different ROS/RNS production rates calculated from currents in D and compared to its values at t =0; (f) time variations of the production rates of O2·– and NO. Adapted with permission from Ref. 47 Copyright 2019 American Chemical Society.