Fig. 2.

Developmental PCB exposure does not alter apoptosis in the bladder. Mice were exposed to PCBs via the maternal diet throughout gestation and lactation. Bladders were collected from male and female offspring at postnatal day (P) 28–31 for immunohistochemistry. Representative images of (A) male and (B) female mouse bladders from each PCB dose group incubated with antibodies targeting cleaved caspase 3 (red) to label apoptotic cells, keratin 5 (KRT5, green) to label basal epithelium and DAPI (blue) to stain nuclei. (C-D) Quantification of the percentage of total (stromal and epithelial) cells that were immunopositive for cleaved caspase-3. Results are mean ± SEM, n = 4–6 bladders per group. One-way ANOVA revealed no significant differences. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)