Fig. 3.

PCBs do not alter bladder epithelial cell composition in developmentally exposed mice. Mice were exposed to PCBs via the maternal diet throughout gestation and lactation and bladders collected from male and female offspring at postnatal day (P) 28–31 for immunohistochemistry. Representative images of (A) male and (B) female mouse bladders from each PCB dose group incubated with antibodies targeting keratin 5 (KRT5, green) to label basal epithelium, transformation related protein 63 (P63, red) to label basal + intermediate epithelium and DAPI (blue) to stain nuclei. Inset illustrates a cell of each type; basal indicated by the yellow arrowhead, intermediate indicated by the orange arrowhead, and superficial indicated by the white arrowhead. Quantification of the percentage of total epithelial cells that express (C-D) both KRT5 and P63 (basal), (E-F) P63 alone (intermediate), or (G-H) neither KRT5 nor P63 (superficial). Results are mean ± SEM, n = 4–6 bladders per group. (C, E, H) One-way ANOVA or (D, G) Welch’s one-way ANOVA or (F) Kruskal-Wallis tests revealed no significant differences. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)