Fig. 5.

PCBs do not alter bladder epithelial cell thickness in developmentally exposed mice. Mice were exposed to PCBs via the maternal diet throughout gestation and lactation and bladders collected from male and female offspring at postnatal day (P) 28–31 for immunohistochemistry. Representative images of (A) male and (B) female mouse bladders from each PCB exposure group incubated with antibodies targeting keratin 5 (KRT5, green) to label basal epithelium, keratin 20 (KRT20, red) to label superficial epithelium and DAPI (blue) to stain nuclei. Quantification of epithelial thickness (microns) of cells that express (C-D) KRT5, (E-F) KRT20, or (G-H) total epithelium KRT5 + KRT20 layers. Results are mean ± SEM, n = 3–6 bladders per group. (D, F, H) One-way ANOVA or (C, E, G) Kruskal-Wallis test revealed no significant differences. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)