Fig. 8.

Increased mast cells in bladder of mice developmentally exposed to PCBs at 6 mg/kg vs 0.1 mg/kg. Mice were exposed to PCBs via the maternal diet throughout gestation and lactation and bladders collected from male and female offspring at postnatal day (P) 28–31. Representative images of (A) male and (B) female mouse bladders from each PCB dose group stained with toluidine blue to identify mast cells (purple). Quantification of (C-D) total number of mast cells normalized to bladder area, (E-F) non-degranulated mast cells normalized to bladder area, (G-H) degranulated mast cells normalized to bladder area. Results are mean ± SEM, n = 4–6 bladders per group. (C, D, F-H) One-way ANOVA or (E) Kruskal-Wallis test were used to determine significant differences. Bar and * indicate significant differences as determined using one-way ANOVA followed by Tukey’s multiple comparisons tests, p ≤ 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)