Table 2.
Antibodies used in this study.
| Primary Antibodies | Source | Company | Catalog # | Dilution |
|---|---|---|---|---|
| Beta-III tubulin | Mouse | Abcam | ab78078 | 1:250 |
| Calcitonin gene related peptide (CGRP) | Mouse | Abcam | ab81887 | 1:200 |
| E-cadherin (CDH1) | Rabbit | Cell Signaling | 3195s | 1:250 |
| Keratin 5 | Chicken | Biologend | 905901 | 1:500 |
| Keratin 20 | Mouse | Dako | M701929-2 | 1:300 |
| Transformation related protein 63 (P63) (4A4) | Mouse | Biocare Medical | CM163 A | 1:200 |
| Uroplakin III | Mouse | Fitzgerald | 10R-U103a | 1:50 |
| Cleaved caspase 3 (Asp 175) | Rabbit | Cell Signaling Technology | 9661S | 1:200 |
| Secondary Antibodies | Paired with primary antibody | ||||
| Anti-Mouse Alexa Fluor 488 | Donkey | Jackson ImmunoResearch | 715-585-150 | 1:250 | Beta-III tubulin |
| Anti-Mouse Alexa Fluor 594 | Donkey | Jackson ImmunoResearch | 715-545-150 | 1:250 | P63, Keratin 20, Uroplakin III, CGRP |
| Anti-Rabbit Alexa Fluor 488 | Donkey | Jackson ImmunoResearch | 711-545-152 | 1:250 | E-cadherin |
| Anti-Rabbit Alexa Fluor 594 | Goat | Jackson ImmunoResearch | 111-516-045 | 1:250 | Cleaved caspase 3, E-cadherin |
| Anti-Chicken Alexa Fluor 488 | Donkey | Jackson ImmunoResearch | 703-546-155 | 1:250 | Keratin 5 |